Cancer Cells in Culture

Normal cells pass through a limited number of cell divisions (70 is about the limit for cells harvested from young animals) before they decline in vigor and die. This is called replicative senescence. It may be caused by their inability to synthesize telomerase.

Cancer cells may be immortal; that is, proliferate indefinitely in culture.

Example: HeLa cells are cultured in laboratories around the world. They are all descended from cells removed from a cancer (of the cervix) of Henrietta Lacks who died of her disease in 1951. (In 2013, DNA sequencing of HeLa cells revealed that human papilloma virus 18 (HPV-18) had integrated upstream of the potent oncogene MYC. [More])

Cancer cells in culture produce telomerase.

Normal cells: when cultured in a dish, they proliferate until the surface of the dish is covered by a single layer of cells just touching each other. Then mitosis ceases. This phenomenon is called contact inhibition.

Cancer cells show no contact inhibition. Once the surface of the dish is covered, the cells continue to divide, piling up into mounds.

The photographs (courtesy of G. Steven Martin) show mouse fibroblasts (connective tissue cells) growing in culture. The cells in the top photo show contact inhibition. Those below do not. The cells below are said to be transformed. These cells (called 3T3 cells) were not derived from a mouse cancer but were produced by laboratory treatment of normal cells. Radiation, certain chemicals, and certain viruses are capable of transforming cells. Although transformed cells are not derived from cancers, they can often develop into malignant tumors when injected into an appropriate test animal (like a nude mouse).

Normal cells are exceedingly fussy about the nutrients that must be supplied to them in their culture medium.

Cancer cells (and transformed cells) can usually grow on much simpler culture medium.

Normal cells ordinarily have the normal set of chromosomes of the species; that is, have a normal karyotype.

Cancer cells almost always have an abnormal karyotype with

• abnormal numbers of chromosomes (polyploid or aneuploid)
• chromosomes with abnormal structure:
  • translocations
  • deletions
  • duplications
  • inversions

Links to three examples of cancers associated with translocations.